Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme

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Published: Oct 31, 2006
DOI: https://doi.org/10.26850/1678-4618eqj.v31.3.2006.p61-65
Keywords:
Pothomorphe umbellata (L.), cell culture, peroxidase

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Najeh Maissar Khalil
Universidade Estadual Paulista, Instituto de Química, Araraquara, Brasil. Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, Araraquara, Brasil.
Miriam Alves Morais Mello
Universidade Estadual Paulista, Instituto de Química, Araraquara, Brasil.
Suzelei de Castro França
Universidade de Ribeirão Preto, Departamento de Biotecnologia, Ribeirão Preto, Brasil.
Luiz Antonio Andrade de Oliveira
Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, Araraquara, Brasil.
Olga Maria Mascarenhas de Faria Oliveira
Universidade Estadual Paulista, Instituto de Química, Araraquara, Brasil.

Abstract

Pothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal
use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity
(units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different
MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth
rate curve showed that the best growth indices were observed when media 2 submitted to the photoperi-
od regime was used, followed by the same media kept in darkness (stress condition). The results obtained
also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxi-
dase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1
were obtained for k M and v max ,, respectively, using guaiacol as enzyme substrate.

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How to Cite
Khalil, N. M., Mello, M. A. M., França, S. de C., de Oliveira, L. A. A., & Oliveira, O. M. M. de F. (2006). Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme. Eclética Química, 31(3), 61–65. https://doi.org/10.26850/1678-4618eqj.v31.3.2006.p61-65
Issue
Vol. 31 No. 3 (2006): Eclética Química
Section
Original articles
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