Keywords
lead
antibiotic
permanent modifier
GFAAS
How to Cite
Abstract
A method has been developed for the simultaneous determination of Cd and Pb in antibiotics used in sugar-cane fermentation by GFAAS. The integrated platform of transversely heated graphite atomizer was treated with tungsten to form a coating of tungsten carbide. Six samples of commercial solid antibiotics were analyzed by injecting 20 μL of digested samples into the pretreated graphite platform with co-injection of 5 μL of 1000 mg L -1 Pd as chemical modifier. Samples were mineralized in a closed-vessel microwave-assisted acid-digestion system using nitric acid plus hydrogen peroxide. The pyrolysis and atomization temperatures of the heating program of the atomizer were selected as 600°C and 2200°C, respectively. The calculated characteristic mass for Cd and Pb was 1.6 pg and 42 pg, respectively. Limits of detection (LOD) based on integrated absorbance were 0.02 μg L -1 Cd and 0.7 μg L -1 Pb and the relative standard deviations (n = 10) for Cd and Pb were 5.7% and 8.0%, respectively. The recoveries of Cd and Pb added to the digested samples varied from 91% to 125% (Cd) and 80% to 112% (Pb).
References
N.V. Narendranath, S.H. Hynes, K.C. Thomas, W.M.
Ingledew, Appl. Environ. Microbiol. 63 (1997) 4158.
P.R.M. Correia, E. Oliveira, P.V. Oliveira, Anal. Chim.
Acta 405 (2000) 205.
V.R.A. Filho, K.G. Fernandes, M. Moraes, J.A.G. Neto,
At. Spectrosc. 23 (2002) 7.
H.M. Kingston, J.B. Jassie, Introduction to microwave
sample preparation – theory and practice, ACS Professional
Reference Book, Washington D.C., 1988.
E. de Oliveira, J. Braz. Chem. Soc. 14 (2003) 174.
A.P. Oliveira, M. Moraes, J.A.G. Neto, E.C. Lima, At.
Spectrosc. 23 (2002) 190.
M.V. Smith, J. Sneddon, Spectrosc. Lett. 32 (1999) 615.
H. Edel, L. Quick, K. Cammann, Anal. Chim. Acta 310
(1995) 181.
A. Viksna, E.S. Lindgren, Anal. Chim. Acta 353 (1997)
A. Deval, J. Sneddon, Microchem. J. 52 (1995) 96.
Jr. Santos, F.J. Barbosa, A.C. Tomazelli, F.J. Krug, J.A.
Nóbrega, M.A.Z. Arruda, Anal. Bioanal. Chem. 373 (2002) 183.
G. P.G. Freschi, C.S. Dakuzaku, M. Moraes, J.A.
Nóbrega, J.A.G. Neto, Spectrochim. Acta B 56 (2001) 1987.
W. Slavin, D.C. Manning, G.R. Carnrick, At. Spectrosc.
(1981) 137.
E.C. Lima, F.J. Krug, K.W. Jackson, Spectrochim. Acta
B 53 (1998) 1791.
E.C. Lima, F. Barbosa, F.J. Krug, J. Anal. At. Spectrom.
(1999) 1913.
G.P.G. Freschi, C.S. Dakuzaku, J.A.G. Neto, M. Moraes,
Eclet. Quím. 25 (2000) 213.
B. Welz, M. Sperling, Atomic Absorption Spectrometry,
rd ed., Wiley-VHC, Weinheim, FRG, 1999.
Recommended conditions for THGA furnaces,PerkinElmer publication B3110.06 Überlingen, Germany
now in Shelton, CT USA, 1991.
H.M.N.H. Irving, H. Freiser, T.S. West, International
Union of Pure and Applied Chemistry: Compendium of
Analytical Nomenclature, Pergamon Press, 1978.
M.L. Cervera, J.C. Lopez, R. Montoro, Microchem. J. 49
(1994) 20.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2018 Eclética Química Journal