A simple method for the spectrophotometric determination of cephalosporins in pharmaceuticals using variamine blue

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Published: Jun 23, 2008
DOI: https://doi.org/10.26850/1678-4618eqj.v33.2.2008.p41-46
Keywords:
cephalosporins determination, spectrophotometry, variamine blue

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Chand Pasha
Mangalore University, Department of Post Graduate Studies and Research in Chemistry, Karnataka, India.
Badiadka Narayana
Mangalore University, Department of Post Graduate Studies and Research in Chemistry, Karnataka, India.
https://orcid.org/0000-0001-8945-4516

Abstract

A simple spectrophotometric method for the determination of cefotaxime, ceftriaxone, cefadroxil and cephalexin with variamine blue is presented. The determination is based on the hydrolysis of β-lactam ring of cephalosporins with sodium hydroxide which subsequently reacts with iodate to liberate iodine in acidic medium. The liberated iodine oxidizes variamine blue to violet colored species of maximum absorption at 556 nm. The absorbance is measured within the pH range of 4.0-4.2. Beer’s law is obeyed in the range of 0.5-5.8 μg mL–1, 0.2-7.0 μg mL–1, 0.2-5.0 μg mL–1 and 0.5-8.5 μg mL–1 for cefotaxime, ceftriaxone, cefadroxil and cephalexin respectively. The analytical parameters were optimized and the method is successfully applied for the determination of cefotaxime, ceftriaxone, cefadroxil and cephalexin in pharmaceuticals.

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How to Cite
Pasha, C., & Narayana, B. (2008). A simple method for the spectrophotometric determination of cephalosporins in pharmaceuticals using variamine blue. Eclética Química, 33(2), 41–46. https://doi.org/10.26850/1678-4618eqj.v33.2.2008.p41-46
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Vol. 33 No. 2 (2008): Eclética Química
Section
Original articles
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