Abstract
The Rangpur lime (Citrus x limonia) is a common fruit in Brazil. Although it is popular in medicine and cuisine, few investigations were conducted into the compositions and antioxidant capacities of infusions, decoctions and from the juice, leaves, and peels. The Folin-Ciocalteu reagent was used to evaluate phenolic content; aluminum chloride to measure flavonols; and sodium borohydride was used to determine flavanones. Antioxidant capacity was assessed through ABTS•+, DPPH• radical scavenging, and ferric ions reducing power. The evaluation of different extracts confirmed that ripe fruit peels contain more phenolics and flavonoids than unripe fruit peels. Furthermore, leaves decoction presents more flavonols and flavones and higher antioxidant activity than infusion and peels, both decoction and infusion. Flavonols were identified as the primary flavonoids in peel and leaf extracts, but no flavanones were found. Conversely, juices displayed a high amount of flavanones, a significant phenolic level, and the best antioxidant capabilities, the most beneficial form for widespread consumption. Further studies must identify the primary compounds in both juices and infusions.
References
Amorim, J.; Simas, D.; Pinheiro, M.; Moreno, D.; Alviano, C.; da Silva, A.; Dias Fernandes, P. Anti-Inflammatory Properties and Chemical Characterization of the Essential Oils of Four Citrus Species. PLoS One. 2016, 11, e0153643. https://doi.org/10.1371/journal.pone.0153643
Apak, R.; Güçlü, K.; Özyürek, M.; Çelik, S. Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchim. Acta. 2007, 160, 413–419. https://doi.org/10.1007/s00604-007-0777-0
Berker, K.; Güçlü, K.; Tor, İ.; Apak, R. Comparative evaluation of Fe(III) reducing power-based antioxidant capacity assays in the presence of phenanthroline, batho-phenanthroline, tripyridyltriazine (FRAP), and ferricyanide reagents. Talanta. 2007, 72, 1157–1165. https://doi.org/10.1016/j.talanta.2007.01.019
Brand-Williams, W.; Cuvelier, M.; Berset, C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci. Technol. 1995, 28, 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
Cavalcanti, E.; Morais, S.; Lima, M.; Santana, E. Larvicidal Activity of essential oils from Brazilian plants against Aedes aegypti L. Mem. Inst. Oswaldo Cruz. 2004, 99, 541–544. https://doi.org/10.1590/S0074-02762004000500015
Chatzianagnostou, K.; Del Turco, S.; Pingitore, A.; Sabatino, L.; Vassalle, C. The Mediterranean Lifestyle as a Non-Pharmacological and Natural Antioxidant for Healthy Aging. Antioxidants. 2015, 4, 719–736. https://doi.org/10.3390/antiox4040719
Chen, Y.; Xiao, H.; Zheng, J.; Liang, G. Structure-Thermodynamics-Antioxidant Activity Relationships of Selected Natural Phenolic Acids and Derivatives: An Experimental and Theoretical Evaluation. PLoS One. 2015, 10, 1–20. https://doi.org/10.1371/journal.pone.0121276
Couto, M. A. L.; Canniatti-Brazaca, S. G. Quantificação de vitamina C e capacidade antioxidante de variedades cítricas. Ciênc. Tecnol. Aliment. 2010, 30 (Suppl. 1), 15-19. https://doi.org/10.1590/S0101-20612010000500003
Curk, F.; Ollitrault, F.; Garcia-Lor, A.; Luro, F.; Navarro, L.; Ollitrault, P. Phylogenetic origin of limes and lemons revealed by cytoplasmic and nuclear markers. Ann. Bot. 2016, 117, 565-583. https://doi.org/10.1093/aob/mcw005
Dong, X.; Hu, Y.; Li, Y.; Zhou, Z. The maturity degree, phenolic compounds and antioxidant activity of Eureka lemon [Citrus limon (L.) Burm. f.]: A negative correlation between total phenolic content, antioxidant capacity and soluble solid content. Sci. Hortic. 2019, 243, 281–289. https://doi.org/10.1016/j.scienta.2018.08.036
Estevam, E. B. B.; Miranda, M. L. D.; Alves, J. M.; Egea, M. B.; Pereira, P. S.; Martins, C. H. G.; Esperandim, V. R.; Magalhães, L. G.; Bolela, A. C.; Cazal, C. M.; Souza, A. F.; Alves, C. C. F. Chemical Composition and Biological Activities of the Essential Oils from the Fresh Leaves of Citrus limonia Osbeck and Citrus latifolia Tanaka (Rutaceae). Rev. Virtual Quím. 2016, 8 (6), 1842–1854. http://doi.org/10.21577/1984-6835.20160124
Fagodia, S.; Singh, H.; Batish, D.; Kohli, R. Phytotoxicity and cytotoxicity of Citrus aurantiifolia essential oil and its major constituents: Limonene and citral. Ind. Crops Prod. 2017, 108, 708–715. http://doi.org/10.1016/j.indcrop.2017.07.005
Fancello, F.; Petretto, G.; Zara, S.; Sanna, M.; Addis, R.; Maldini, M.; Foddai, M.; Rourke, J.; Chessa, M.; Pintore, G. Chemical characterization, antioxidant capacity and antimicrobial activity against food-related microorganisms of Citrus limon var. pompia leaf essential oil. LWT - Food Sci. Technol. 2016, 69, 579–585. https://doi.org/10.1016/j.lwt.2016.02.018
Food and Agriculture Organization of the United Nations (FAOSTAT). Crops and livestock products. http://www.fao.org/faostat/en/#data/QC (accessed Mar 1, 2020).
Floegel, A.; Kim, D.; Chung, S.; Koo, S.; Chun, O. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J. Food Compos. Anal. 2011, 24, 1043–1048. https://doi.org/10.1016/j.jfca.2011.01.008
Fotakis, C.; Tsigrimani, D.; Tsiaka, T.; Lantzouraki, D.; Strati, I.; Makris, C.; Tagkouli, D.; Proestos, C.; Sinanoglou, V.; Zoumpoulakis, P. Metabolic and antioxidant profiles of herbal infusions and decoctions. Food Chem. 2016, 211, 963–971. https://doi.org/10.1016/j.foodchem.2016.05.124
Freitas, A.; Coelho, M.; Pereira, Y.; Freitas Neto, E.; Azevedo, R. Diversidade e usos de plantas medicinais nos quintais da comunidade de São João da Várzea em Mossoró, RN. Rev. Bras. Plantas Med. 2015, 17, 845–856. https://doi.org/10.1590/1983-084X/14_080
Gattuso, G.; Barreca, D.; Gargiulli, C.; Leuzzi, U.; Caristi, C. Flavonoid Composition of Citrus Juices. Molecules. 2007, 12, 1641–1673. http://doi.org/10.3390/12081641
Geronikaki, A.; Gavalas, A. Antioxidants and Inflammatory Disease: Synthetic and Natural Antioxidants with Anti-Inflammatory Activity. Comb. Chem. High Throughput Screen. 2006, 9, 425–442. https://doi.org/10.2174/138620706777698481
González-Mas, M. C.; Rambla, J. L.; López-Gresa, M. P.; Blázquez, M. A.; Granell A. Volatile Compounds in Citrus Essential Oils: A Comprehensive Review. Front. Plant Sci. 2019, 10, 1–18. https://doi.org/10.3389/fpls.2019.00012
Huang, D.; Ou, B.; Prior, R. The Chemistry behind Antioxidant Capacity Assays. J. Agric. Food Chem. 2005, 53, 1841–1856. https://doi.org/10.1021/jf030723c
Huynh, D. T.; Vo, M. T. N.; Kha, T. C. Enriching the Bioactive Components and Antioxidant Capacity of Concentrated Lime Juices Prepared by Cryogenic and Vacuum Processes. Processes. 2023, 11, 1883. https://doi.org/ 10.3390/pr11071883
Jeong, S.; Kim, S.; Kim, D.; Jo, S.; Nam, K.; Ahn, D.; Lee, S. Effect of Heat Treatment on the Antioxidant Activity of Extracts from Citrus Peels. J. Agric. Food Chem. 2004, 52, 3389–3393. https://doi.org/10.1021/jf049899k
Kimball, D. Citrus Processing; Springer Netherlands: Dordrecht, 1991; pp. 117–125. https://doi.org/10.1007/978-94-011-3700-3_8
Lagha-Benamrouche, S.; Madani, K. Phenolic contents and antioxidant activity of orange varieties (Citrus sinensis L. and Citrus aurantium L.) cultivated in Algeria: Peels and leaves. Ind. Crops Prod. 2013, 50, 723–730. http://dx.doi.org/10.1016/j.indcrop.2013.07.048
Letaief, H.; Zemni, H.; Mliki, A.; Chebil, S. Composition of Citrus sinensis (L.) Osbeck cv «Maltaise demi-sanguine» juice. A comparison between organic and conventional farming. Food Chem. 2016, 194, 290–295. https://doi.org/10.1016/j.foodchem.2015.08.025
Loizzo, M.; Tundis, R.; Bonesi, M.; Menichini, F.; De Luca, D.; Colica, C.; Menichini, F. Evaluation of Citrus aurantifolia peel and leaves extracts for their chemical composition, antioxidant and anti-cholinesterase activities. J. Sci. Food Agric. 2012, 92, 2960–2967. https://doi.org/10.1002/jsfa.5708
Martins, M.; Fracarolli, L.; Vieira, T.; Dias, H.; Cruz, M.; Deus, C.; Nicolella, H.; Stefani, R.; Rodrigues, V.; Tavares, D.; Magalhães, L.; Crotti, A. Schistosomicidal Effects of the Essential Oils of Citrus limonia and Citrus reticulata Against Schistosoma mansoni. Chem. Biodivers. 2016, 14, e1600194. https://doi.org/10.1002/cbdv.201600194
Mcharek, N.; Hanchi, B. Maturational effects on phenolic constituents, antioxidant activities and LC-MS / MS profiles of lemon Citrus limon peels. J. Appl. Bot. Food Qual. 2017, 90, 1–9. https://doi.org/10.5073/JABFQ.2017.090.001
Mehmood, T.; Afzal, A.; Anwar, F.; Memon, N.; Memon, A. A.; Qadir, R. Variation in phenolic acids and antibacterial attributes of peel extracts from ripe and unripe [Citrus limon (L.) Osbeck] fruit. J. Food Meas. Charact. 2020, 14,1325–1332. https://doi.org/10.1007/s11694-020-00380-w
Millezi, A.; Baptista, N.; Caixeta, D.; Rossoni, D.; Cardoso, M.; Piccoli, R. Caracterização química e atividade antibacteriana de óleos essenciais de plantas condimentares e medicinais contra Staphylococcus aureus e Escherichia coli. Rev. Bras. Plantas Med. 2014, 16, 18–24. https://doi.org/10.1590/S1516-05722014000100003
Moriguchi, T.; Kita, M.; Tomono, Y.; Endo-Inagaki, T.; Omura, M. Gene expression in flavonoid biosynthesis: Correlation with flavonoid accumulation in developing citrus fruit. Physiol. Plant. 2001, 111, 66–74. https://doi.org/10.1034/j.1399-3054.2001.1110109.x
Muthiah, P.; Umamaheswari, M.; Asokkumar, K. In vitro antioxidant activities of leaves, fruits and peel extracts of citrus. Int. J. Phytopharm. 2012, 2, 13–20. https://doi.org/10.7439/ijpp.v2i1.293
Nogata, Y.; Sakamoto, K.; Shiratsuchi, H.; Ishii, T.; Yano, M.; Ohta, H. Flavonoid Composition of Fruit Tissues of Citrus Species. Biosci. Biotechnol. Biochem. 2006, 70, 178–192. https://doi.org/10.1271/bbb.70.178
Othman, S.; Hassan, M.; Nahar, L.; Basar, N.; Jamil, S.; Sarker, S. Essential Oils from the Malaysian Citrus (Rutaceae) Medicinal Plants. Medicin. 2016, 3, 13. https://doi.org/10.3390/medicines3020013
Panahi, Y.; Rajaee, S.; Johnston, T.; Sahebkar, A. Neuroprotective effects of antioxidants in the management of neurodegenerative disorders: A literature review. J. Cell. Biochem. 2018, 120, 2742–2748. https://doi.org/10.1002/jcb.26536
Pękal, A.; Pyrzynska, K. Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay. Food Anal. Methods. 2014, 7, 1776–1782. https://doi.org/10.1007/s12161-014-9814-x
Penjor, T.; Yamamoto, M.; Uehara, M.; Ide, M.; Matsumoto, N.; Matsumoto, R.; Nagano, Y. Phylogenetic Relationships of Citrus and Its Relatives Based on matK Gene Sequences. PLoS One. 2013, 8, e62574. https://doi.org/10.1371/journal.pone.0062574
Rafiq, S.; Kaul, R.; Sofi, S.; Bashir, N.; Nazir, F.; Ahmad Nayik, G. Citrus peel as a source of functional ingredient: A review. J. Saudi Soc. Agric. Sci. 2018, 17, 351–358. https://doi.org/10.1016/j.jssas.2016.07.006
Rahmiati, N.; Sari, R.; Wahyuni, S. T.; Lestari, M. L. A. D. Enhancing antioxidant properties of lime juice powder through polyelectrolyte microparticles of chitosan-alginate: Formulation, characterization and stability study. J. Adv. Pharm. Technol. Res. 2024, 15, 231-236. https://doi.org/10.4103/JAPTR.JAPTR_556_23
Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 1999, 26, 1231–1237. https://doi.org/10.1016/S0891-5849(98)00315-3
Rowell, K.; Winter, D. Quantitative Determination of Flavanones in Citrus Bioflavonoids by Potassium Borohydride Reduction. J. Am. Pharm. Assoc. 1959, 48, 746–749. https://doi.org/10.1002/jps.3030481214
Sanches, V. L.; Cunha, T. A.; Viganó, J.; Mesquita, L. M. S.; Faccioli, L. H.; Breitkreitz, M. C.; Rostagno, M. A. Comprehensive analysis of phenolics compounds in citrus fruits peels by UPLC-PDA and UPLC-Q/TOF MS using a fused-core column. Food Chem. 2022, 14, 1–9. https://doi.org/10.1016/j.fochx.2022.100262
Schäfer, G.; Bastianel, M.; Dornelles, A. Porta-enxertos utilizados na citricultura. Ciência Rural. 2001, 31, 723–733. http://hdl.handle.net/10183/22374
Schaich, K.; Tian, X.; Xie, J. Hurdles and pitfalls in measuring antioxidant efficacy: A critical evaluation of ABTS, DPPH, and ORAC assays. J. Funct. Foods. 2015, 14, 111–125. https://doi.org/10.1016/j.jff.2015.01.043
Sentkowska, A.; Biesaga, M.; Pyrzynska, K. Effects of brewing process on phenolic compounds and antioxidant activity of herbs. Food Sci. Biotechnol. 2016, 25, 965–970. https://doi.org/10.1080/10942912.2014.960932
Sentkowska, A.; Biesaga, M.; Pyrzynska, K. Polyphenolic Composition and Antioxidative Properties of Lemon Balm (Melissa officinalis L.) Extract Affected by Different Brewing Processes. Int. J. Food Prop. 2014, 18, 2009–2014. https://doi.org/10.1007/s10068-016-0157-9
Siddhartha, A.; Kaur, H.; Bains, K.; Kaur S. Comparative Analysis of Bioactive Compounds and Antioxidant Potential Among Various Citrus Varieties and Their Juices. Appl. Fruit Sci. 2025, 67, 31. https://doi.org/10.1007/s10341-024-01251-0
Synowiec-Wojtarowicz, A.; Kiselova, Y.; Yanov, D.; Ivanova, D.; Bialik, W.; Kimsa-Dudek, M.; Pawłowska–Góral, K. Effects of polyphenol supplementation to in vitro antioxidant potential of citrus juices. Scripta Sci. Pharm. 2018, 5, 28–32. http://doi.org/10.14748/ssp.v1i1.5036
Wang, H.; Chen, G.; Guo, X.; Abbasi, A.; Liu, R. Influence of the stage of ripeness on the phytochemical profiles, antioxidant and antiproliferative activities in different parts of Citrus reticulata Blanco cv. Chachiensis. LWT - Food Sci. Technol. 2016, 69, 67–75. https://doi.org/10.1016/j.lwt.2016.01.021
Yoo, K.; Lee, K.; Park, J.; Lee, H.; Hwang, I. Variation in Major Antioxidants and Total Antioxidant Activity of Yuzu (Citrus junosSieb ex Tanaka) during Maturation and between Cultivars. J. Agric. Food Chem. 2004, 52, 5907–5913. https://doi.org/10.1021/jf0498158
Zhang, Y.; Gan, R.; Li, S.; Zhou, Y.; Li, A.; Xu, D.; Li, H. Antioxidant Phytochemicals for the Prevention and Treatment of Chronic Diseases. Molecules. 2015, 20, 21138–21156. https://doi.org/10.3390/molecules201219753
Zhang, Y.; Sun, Y.; Xi, W.; Shen, Y.; Qiao, L.; Zhong, L.; Ye, X.; Zhou, Z. Phenolic compositions and antioxidant capacities of Chinese wild mandarin (Citrus reticulata Blanco) fruits. Food Chem. 2014, 145, 674-680. https://doi.org/10.3390/molecules201219753
Zou, Z.; Xi, W.; Hu, Y.; Nie, C.; Zhou, Z. Antioxidant activity of Citrus fruits. Food Chem. 2016, 196, 885–896. https://doi.org/10.1016/j.foodchem.2015.09.072

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