Larvicidal activity, molluscicide and toxicity of the essential oil of Citrus limon peels against, respectively, Aedes aegypti, Biomphalaria glabrata and Artemia salina
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Abstract
In this present work, we tested the larvicidal activity, molluscicide and toxicity of the oil extracted from Citrus limon peels, respectively against third stage larvae of Aedes aegypti, snail Biomphalaria glabrata, and Artemia salina. For this, we extract the essential oil by hydrodistillation. Then, we identified and quantified the components by gas chromatography coupled to mass spectrometry (GC-MS). We tested the larvicidal and molluscicidal activity, respectively, using the method adopted by the Brazilian Ministry of Health and the World Health Organization. We calculated the lethal concentration (LC50) from the Probit method for the three biological activities with 95 %. The results of the chromatographic analysis showed that the oil has 58.81% of Limonene (major constituent) and 0.11% α-Mulene (minority component). The essential oil presented lethal concentration (LC50) for larvicidal activity, molluscicide and toxicity, respectively at 15.48, 13.05 and 743.35 mg∙L-1. Therefore, the essential oil is active against larvae of A. aegypti and snail B. glabrata and non-toxic against larvae of A. salina.
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References
Buettner, A., Mestres, M., Fischer, A., Guasch, J., Schieberle, P., Evaluation of the most odour-active compounds in the peel oil of clementines (Citrus reticulata Blanco cv. Clementine), Eur Food Res. Technol. 216 (1) (2003) 11-14. https://doi.org/10.1007/s00217-002-0586-y.
Caccioni, D. R., Guizzardi, M., Biondi, D. M., Agatino, R., Ruberto, G., Relationship between volatile components of citrus fruit essential oils and antimicrobial action on Penicillium digitatum and Penicillium italicum, Int J Food Microbiol 43 (1-2) (1998) 73-79. https://doi.org/10.1016/S0168-1605(98)00099-3.
Sharma, N., Tripathi, A., Fungitoxicity of the essential oil of Citrus sinensis on post-harvest pathogens, World J. Microbiol. Biotechnol. 22 (6) (2006) 587-593. https://doi.org/10.1007/s11274-005-9075-3.
Demyttenaere, J., De Kimpe, N., Biotransformation of terpenes by fungi: Study of the pathways involved, J. Mol. Catal. B Enzym. 11 (4) (2001) 265-270. https://doi,org/10.1016/S1381-1177(00)00040-0.
Martins, G. D. S. O., Zago, H. B., Costa, A. V., Araujo Junior, L. M. D., Carvalho, J. R. D., Chemical composition and toxicity of citrus essential oils on Dysmicoccus brevipes (Hemiptera: Pseudococcidae), Rev. Caatinga 30 (3) (2017) 811-817. https://doi.org/10.1590/1983-21252017v30n330rc.
Kumar, P., Singh, D. K., Molluscicidal activity of Ferula asafoetida, Syzygium aromaticum and Carum carvi and their active components against the snail Lymnaea acuminata, Chemosphere 63 (9) (2006) 1568-1574. https://doi.org/10.1016/j.chemosphere.2005.08.071.
Cantanhede, S. P. D., Marques, A. M., Silva-Souza, N., Valverde, A. L., Atividade moluscicida de plantas: uma alternativa profilática, Rev. Bras. Farmacogn. 20 (2) (2010) 282-288. https://doi.org/10.1590/S0102-695X2010000200024.
Colley, D.G., Bustinduy, A. L., Secor, W. E., King, C. H., Human schistosomiasis, The Lancet 383 (9936) (2014) 2253-2264. https://doi.org/10.1016/S0140-6736(13)61949-2.
Gasparotto Junior., A., Brenzan, M. A., Piloto, I. C., Cortez, D. A. G., Nakamura, C. V., Dias Filho, B. P., Rodrigues Filho, E., Ferreira, A. G., Estudo fitoquímico e avaliação da atividade moluscicida do Calophyllum brasiliense Camb (Clusiaceae), Quím Nova 28 (4) (2005) 575-578. https://doi.org/10.1590/S0100-40422005000400003.
Diniz, M. M. C. S., Henriques, A. D. S., Leandro, R. S., Aguiar, D. L., Beserra, E. B., Resistance of Aedes aegypti to temephos and adaptive disadvantages, Rev Saúde Publica 48 (2014) 775-782. https://doi.org/10.1590/S0034-8910.2014048004649.
World Health Organization, Report of the Scientific working Group on Plant Molluscicide & Guidelines for evaluation of plant molluscicides, Bull World Health Organ Geneva TDRSC (1983). https://apps.who.int/iris/handle/10665/60086.
Utyama, I. K, A., Andrade, D., Watanabe, E., Pimenta, F. C., Ito, I. Y., Determinação da atividade antibacteriana e toxicidade do ácido acético e vinagres branco e tinto, Revista Eletrônica de Farmácia 4 (2) (2007) 202-207. https://doi.org/10.5216/ref.v4i2.3054.
Calow, P., Marine and estuarine invertebrate toxicity tests, Hoffman Al Handb Cytotoxicology, Oxford, Blackwell, Sci Publication, 1993.
Singh, A., Singh, S. K., Molluscicidal evaluation of three common plants from India, Fitoterapia 76 (7-8) (2005) 747-751. https://doi.org/10.1016/j.fitote.2005.08.002.
Luna, J. S., Santos, A., Lima, M. R. F., Omena, M. C., Mendonça, F. A. C., Bieber, L. W., Sant’Ana, A. E. G., A study of the larvicidal and molluscicidal activities of some medicinal plants from northeast Brazil, J. Ethnopharmacol. 97 (2) (2005) 199-206. https://doi.org/10.1016/j.jep.2004.10.004.
Adams, R. P., Sparkman, O., Review of identification of essential oil components by gas chromatography/mass spectrometry, J. Am. Soc. Mass Spectrom 18 (4) (2007) 803-806. https://doi.org/10.1016/j.jasms.2007.01.001.
Brasil, Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância Epidemiológica, Vigilância e controle de moluscos de importância epidemiológica: diretrizes técnicas: programa de vigilância e controle da esquistossomose (PCE), Brasília: Ministério da Saúde, 2008. http://portalarquivos.saude.gov.br/images/pdf/2015/agosto/14/vigilancia-controle-moluscos-import-epidemio-2ed.pdf.
Deslandes, N., Técnica de dissecação e exame de planorbídeos, Rev Serv Espec Saúde Pública 4 (1951) 371-382.
Smithers, S. R., Terry, R., The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms, Parasitology, 55 (4) (1965) 695-700. https://doi.org/10.1017/s0031182000086248.
Malek, E. A., Snail Hosts of Schistosomiasis and Other Snail-Transmitted Diseases in Tropical America: a manual, American Society of Tropical Medicine and Hygiene, Arlington, 1985. https://doi.org/10.4269/ajtmh.1987.36.199.
McCullough, F. S., Gayral, P., Duncan, J., Christie, J. D., Molluscicides in schistosomiasis control, Bull. World Health Organ. 58 (5) (1980) 681-689. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395986/.
Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E., McLaughlin, J. L., Brine shrimp: a convenient general bioassay for active plant constituents, Planta Med. 45 (1) (1982) 31-34. https://doi.org/10.1055/s-2007-971236.
Amarante, C. B. D., Müller, A. H., Póvoa, M. M., Dolabela, M. F., Phytochemical study bioassay-guided by tests of toxicity on Artemia salina and antiplasmodial activity from stem of aninga (Montrichardia linifera), Acta Amazonica, 41 (3) (2011) 431-434. https://doi.org/10.1590/S0044-59672011000300015.
Finney, D.J., Tattersfield, F., Probit Analysis, Cambridge University: Cambridge, 1952.
Mouchrek Filho, V. E., Estudos Analíticos e modificações químicas por metilação e acetilação do eugenol contido no óleo essencial extraído das folhas da espécie Pimenta dioica Lindl, Tese de Doutorado, Universidade de São Paulo, São Paulo, Brasil, 2000.
Gomes, P. R. B., Silva, A. L. S., Pinheiro, H. A., Carvalho, L. L., Lima, H. S., Silva, E. F., Silva, R. P., Louzeiro, H.C., Oliveira, M. B., Mouchrek Filho, V. E., Avaliação da atividade larvicida do óleo essencial do Zingiber officinale Roscoe (gengibre) frente ao mosquito Aedes aegypti, Rev. Bras. Plantas Med. 18 (2) (2016) 597-604. https://doi.org/10.1590/1983-084x/15_214.
Gomes, P. R. B., Oliveira, R. W. S., Mouchrek Filho, V. E., Nascimento, A. A., Everton, A. P., Louzeiro, H. C., Fontenele, M. A., Activity larvicide of the essential oil Syzygium aromaticum (carnival-of-India) in front of the mosquito Aedes aegypti (Linnaeus, 1762). Periodico Tche Quimica 15 (29) (2018) 184-195. http://www.deboni.he.com.br/Periodico29.pdf.
Gomes, P. R. B., Santos, D. P., Mouchrek Filho, V. E., Mendes, L. S. S., Fontenele, M. A., Activity larvicide of the essential oil of Cinnamomum zeylanicum Blume, Period Tche Quimica 16 (31) (2019) 18-26. http://www.deboni.he.com.br/Periodico31.pdf.
Gomes, P. R. B., Silva, A. L. S., Mouchrek Filho, V. E., Mouchrek, A. N., Everton, P. C., Avaliação físico-química do óleo essencial Zingiber officinale Roscoe (Gengibre), Rev. Cuba Farm. 50 (3) (2016). http://www.revfarmacia.sld.cu/index.php/far/article/view/30/34.
Gomes, P. R. B., Mouchrek Filho, V. E., Ferreira Rabêlo, W., Nascimento, A. A., Costa Louzeiro, H., Lyra, W. S., Fontenele, M. A., Chemical characterization and cytotoxicity of clove essential oil (Syzygium aromaticum), Rev. Colomb. Cienc. Quím. – Farm. 47 (1) (2018) 37-52. https://doi.org/10.15446/rcciquifa.v47n1.70657.
Campelo, L. M. L., Sá, C. G., Feitosa, C. M., Sousa, G. F., Freitas, R. M., Constituintes químicos e estudos toxicológicos do óleo essencial extraído das folhas de Citrus limon Burn (Rutaceae), Rev. Bras. Plantas Med. 15 (4 suppl 1) (2013) 708-716. https://doi.org/10.1590/S1516-05722013000500011.
González-Molina, E., Domínguez-Perles, R., Moreno, D. A., García-Viguera, C., Natural bioactive compounds of Citrus limon for food and health, J. Pharm. Biomed. Anal. 51 (2) (2010) 327-345. https://doi.org/10.1016/j.jpba.2009.07.027.
Mahalwal, V. S., Ali, M., Volatile constituents of the fruits peels of Citrus lemon (Linn) Burm. f. J. Essent. Oil Bear Plants 6 (1) (2003) 31-35. https://doi.org/10.1080/0972-060X.2003.10643325.
Blank, A. F., Costa, A. G., Arrigoni-Blank, M. F., Cavalcanti, S. C. D. H., Alves, P. B., Innecco, R., Ehlert, P. A. D., Sousa, I. F., Influence of season, harvest time and drying on Java citronella (Cymbopogon winterianus Jowitt) volatile oil, Rev. Bras. Farmacogn. 17 (4) (2007) 557-564. https://doi.org/10.1590/S0102-695X2007000400014.
Figueiredo, A. C., Barroso, J. G., Pedro, L. G., Scheffer, J. J. C., Factors affecting secondary metabolite production in plants: volatile components and essential oils, Flavour Fragr. J. 23 (4) (2008) 213-226. https://doi.org/10.1002/ffj.1875.
Cerqueira, M. D., Marques, E. J., Martins, D., Roque, N. F., Cruz, F. G., Guedes, M. L. S., Seasonal variation of the composition of essential oil from Myrcia salzmannii Berg. (Myrtaceae), Quím Nova 32 (6) (2009) 1544-1548. https://doi.org/10.1590/S0100-40422009000600035.
Dias, C. N., Moraes, D. F. C., Essential oils and their compounds as Aedes aegypti L. (Diptera: Culicidae) larvicides: review, Parasitol Res. 113 (2) (2014) 565-592. https://doi.org/10.1007/s00436-013-3687-6.
Cheng, S., Chang, H. T., Chang, S.T., Tsai, K. H., Chen, W. J., Bioactivity of selected plant essential oils against the yellow fever mosquito Aedes aegypti larvae, Bioresour Technol. 89 (1) (2003) 99-102.https://doi.org/10.1016/S0960-8524(03)00008-7.
Fernandez, C. M. M., Barba, E. L., Fernandez, A. C. M., Cardoso, B. K., Borges, I. B., Takemura, O. S., Martins, L. A., Cortez, L. E. R., Cortez, D. A. G., Gazim, Z. C., Larvicidal activity of essential oil from Tetradenia riparia to control of Aedes aegypti larvae in function of season variation, J. Essent. Oil Bear Plants 17 (5) (2014) 813-823. https://doi.org/10.1080/0972060X.2014.892841.
Leyva, M., Marquetti, M. C., Tacoronte, J. E., Scull, R., Tiomno, O., Mesa, A., Montada, D., Actividad larvicida de aceites esenciales de plantas contra Aedes aegypti (L.) (Diptera: Culicidae), Rev. Biomed. 20 (1) (2009) 5-13. http://revistabiomedica.mx/index.php/revbiomed/article/view/529.
Lee, H. S., Mosquito larvicidal activity of aromatic medicinal plant oils against Aedes aegypti and Culex pipiens pallens, J. Am. Mosq. Control Assoc. 22 (2) (2006) 292-296. https://doi.org/10.2987/8756-971X(2006)22[292:MLAOAM]2.0.CO;2.
Leite, A. M., Lima, E. O., Souza, E. L., Diniz, M. D. F., Leite, S. P., Xavier, A. L., Medeiros, I. A. D., Preliminary study of the molluscicidal and larvicidal properties of some essential oils and phytochemicals from medicinal plants, Rev. Bras. Farmacogn. 19 (4) (2009) 842-846. https://doi.org/10.1590/S0102-695X2009000600008.
Lucia, A., Audino, P. G., Seccacini, E., Licastro, S., Zerba, E., Masuh, H., Larvicidal effect of Eucalyptus grandis essential oil and turpentine and their major components on Aedes aegypti larvae, J. Am. Mosq. Control Assoc. 23 (3) (2007) 299-304. https://doi.org/10.2987/8756-971X(2007)23[299:LEOEGE]2.0.CO;2.
Campolo, O., Romeo, F. V., Algeri, G. M., Laudani, F., Malacrinò, A., Timpanaro, N., Palmeri, V. Larvicidal effects of four citrus peel essential oils against the arbovirus vector Aedes albopictus (Diptera: Culicidae), J. Econ. Entomol. 109 (1) (2015) 360-365. https://doi.org/10.1093/jee/tov270.
Giatropoulos, A., Papachristos, D. P., Kimbaris, A., Koliopoulos, A., Polissiou, M. G., Emmanouel, N., Michaelakis, A., Evaluation of bioefficacy of three Citrus essential oils against the dengue vector Aedes albopictus (Diptera: Culicidae) in correlation to their components enantiomeric distribution, Parasitol. Res. 111 (6) (2012) 2253-2263. https://doi.org/10.1007/s00436-012-3074-8.
Amer, A., Mehlhorn, H., Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae), Parasitol. Res. 99 (4) (2006) 466-472. https://doi.org/10.1007/s00436-006-0182-3.
Petersen, P. E., World Health Organization. Organisation Mondiale de la Sante, Community Dent. Oral Epidemiol. 31 (6) (2003) 471-471. https://doi.org/10.1046/j.1600-0528.2003.00124.x.
Silva, T., Batista, M., Camara, C., Agra, M., Molluscicidal activity of some Brazilian Solanum spp. (Solanaceae) against Biomphalaria glabrata, Ann. Trop. Med. Parasitol. 99 (4) (2005) 419-425. https://doi.org/10.1179/136485905X36208.
Silva, C. L. P. A. C., Vargas, T. S., Baptista, D. F., Molluschicidal activity of Moringa oleifera on Biomphalaria glabrata: integrated dynamics to the control of the snail host of Schistosoma mansoni, Rev. Bras. Farm. 23 (2013) 848-850. https://doi.org/10.1590/S0102-695X2013000500019.
Silva, T., Silva, T., Martins, R., Maia, G. L. A., Cabral, A. G. S., Camara, C. A., Agra, M. F., Barbosa-Filho, J. M., Molluscicidal activities of six species of Bignoniaceae from north–eastern Brazil, as measured against Biomphalaria glabrata under laboratory conditions, Ann. Trop. Med. Parasitol. 101 (4) (2007) 359-365. https://doi.org/10.1179/136485907X176427.
Coutinho, D. F., Dias, C. S., Barbosa-Filho, J. M., Agra, M. F., Martins, R. M., Silva, T. M., Cunha, E. V. L., Silva, M. S., Craveiro, A. A., Composition and molluscicidal activity of the essential oil from the stem bark of Ocotea bracteosa (Meisn.) Mez, J. Essent. Oil Res. 19 (5) (2007) 482-484. https://doi.org/10.1080/10412905.2007.9699958.
Farmacopéia Brasileira, Editora Atheneu, parte I-II, quinto fascículo, São Paulo, 4ª ed., 2004.
Gomes, P. R. B., Reis, J. B., Fernandes, R. P., Mouchrek Filho, V. E., Souza, A. G., Fontenele, M. A., Silva, J. C., Toxicidad y Actividad Molusccidal del Aceite Esencial Pimenta dioica Contra El Caracol Biomphalaria glabrata. Revista Peruana de Biología 26 (1) (2019) 101-8. https://doi.org/10.15381/rpb.v26i1.15913.
Chaar, J. S., Estudos analíticos e modificação química por acetilação do linalol contido no óleo essencial da espécie Aniba duckei Kostermans, Tese de Doutorado, Universidade de São Paulo, São Paulo, Brasil, 2000. https://doi.org/10.11606/T.75.2000.tde-28112001-085626.