Abstract
The present work reports our succesfull experience concerning crystallization of four fish hemoglobins from three Brazilian species of Teleosts: Liposarcus anisitsi, Brycon cephalus and Piaractus mesopotamicus. The data shown here is part of a systematic functional and structural study of fish hemoglobins with the aim of better understanding the outstanding range of functional and structural properties exhibited by these proteins. We also present a reduced sparse-matrix method for crystallization of fish hemoglobins, which can reduce the amount of hemoglobin initially used in the crystallization experiments.
References
DE AZEVEDO, W. F. JR., MUELLER-DIECKMANN, H-. J., SCHULZE-GAHMEN, U., WORLAND, P. J., SAUSVILLE, E., KIM, S-. H. Structural basis for specificity and potency of a flavonoid inhibitor of human CDK2, a cell cycle kinase. Proc. Natl. Acad. Sci. USA. v. 93. p.2735-40, 1996. [ Links ]
DE AZEVEDO, W. F., JR., LECLERC, S., MEOJER, L., HAVLICEK, L., STRNAD, M. and KIM, S. –H. Inhibition of cyclin-dependent kinases by purine analogues: crystal structure of human cdk2 complexed with roscovitine. Eur. J. Biochemistry, v.243, 518-26, 1997. [ Links ]
BLUNDELL, T. L., JOHNSON, L. N. Protein Crystallography. London: Academic Press, 1976. [ Links ]
BRÜNGER, A. T. X-PLOR Version 3.1: A system for crystallography and NMR. New Haven: Yale University Press, 1992. [ Links ]
DELATORRE, P., SMARRA, A. L. S., FADEL, V., CANDURI, F., DELLAMANO, M., BONILLA-RODRIGUEZ, G. O., DE AZEVEDO JR, W. F. Purification, crystallization, and Patterson search of hemoglobin-IV from the armored catfish Liposarcus anisitsi. Acta Crystallographica D. 2000. (In Press). [ Links ]
DE YOUNG, A., KWIATKOWSKI, L. D., NOBLE, R. W. Fish Hemoglobins. Methods of Enzymol. v. 231, p. 124-50, 1994. [ Links ]
DRENTH, J. Principles of protein x-ray crystallography. Berlin: Springer-Verlag, 1994. 305p. [ Links ]
FADEL, V., HONDA, R.T., DELLAMANO, M., SMARRA, A. L. S., DELATORRE, P., OLIVIERI, J. R., BONILLA-RODRIGUEZ, G. O., DE AZEVEDO JR., W. F. Purification, crystallization, and preliminary X-ray diffraction analysis of carbomonoxy hemoglobin-II from the fish Piaractus mesopotamicus (Pacu). Acta Cryst. D56, p.366-7, 2000. [ Links ]
FAGO, A., MALTE, H., DOHN, N. Bicarbonate binding to hemoglobin links oxygen and carbon dioxide transport in hagfish. Resp. Physiol. v. 115, p. 309-15, 1999. [ Links ]
HONDA, R., DELATORRE, P., FADEL, V., CANDURI, F., DELLAMANO, M., DE AZEVEDO JR., W. F., BONILLA-RODRIGUEZ, G. O. Crystallisation, preliminary X-ray analysis, and molecular-replacement solution of Carbomonoxy form of haemoglobin-I from the fish Brycon cephalus. Acta Cryst. D56, 2000. [ Links ](In Press).
KIM. S. -H., SCHULZE-GAHMEN, U., BRANDSEN, J., DE AZEVEDO, W. F., JR. Structural basis for chemical inhibition of CDK2. Progress in Cell Cycle Research, v. 2, 137-145. Chapter 14. (Meijer, L., Guidet, S. & Vogel, L., eds.) Plenum Press, New York, USA., 1996. (Review). [ Links ]
JANCARICK, J. AND KIM, S. H. Sparse matrix sampling – a screening method for crystallization of proteins. J. Appl. Crystallogr. v. 24, p. 409-11, 1991. [ Links ]
McREE, D. E. Pratical Protein Crystallography. San Diego: Academic Press, Inc., 1993. 386p. [ Links ]
MATTHEWS, B. W. Solvent content of protein crystals. J. Mol. Biol. v. 33, p. 491-7, 1968. [ Links ]
NAVAZA, J. AMoRe: An Automated Package for Molecular Replacement. Acta Cryst. A50, p. 157-63, 1994. [ Links ]
OTWINOWSKI, Z. In Proceedings of the CCP4 Study Weekend, edited by L. Sawyer, N. Isaacs & S. Bailey, Warrington: Daresbury Laboratory. p. 56-62. 1993. [ Links ]
POLIKARPOV, I., OLIVA, G., CASTELLANO, E.E., GARRATT, R., ARRUDA, P., LEITE, A., CRAIEVICH, A. Protein crystallography station at LNLS, The Brazilian National Synchrotron Ligth Source. Nucl. Instrum. Methods A, v. 405, p. 159-64, 1998. [ Links ]
POLIKARPOV, I., PERLES, L. A., DE OLIVEIRA, R. T., OLIVA, G., CASTELLANO, E.E., GARRATT, R., CRAIEVICH, A. Set-up and experimental parameters of the protein crystallography beamline at Brazilian National Synchrotron Laboratory. J. Synchrotron Rad. v. 5, p. 72-6, 1998. [ Links ]
RODGERS, D. W. Cryocrystallography. Structure, v. 2, n. 12, p. 1135-1140, 1994. [ Links ]
SEIXAS, F. A., DE AZEVEDO, W. F. JR., COLOMBO, M. F. Crystallization and X-Ray diffraction data analysis of human deoxy-hemoglobin A0 stripped of any anions. Acta Cryst. D55, p. 1914-6, 1999. [ Links ]
SMARRA, A. L. S, ARNI, R. K. DE AZEVEDO, JR., W. F., COLOMBO, M. F., BONILLA-RODRIGUEZ, G. O. Crystallization and X-ray diffraction data analysis of Oxyhemoglobin-I from the Liposarcus anisitsi (Pisces) Protein and Peptide Letters. v. 4, p.349-54, 1997. [ Links ]
SMARRA, A. L. S., FADEL, V., DELLAMANO, M., OLIVIERI, J.R., DE AZEVEDO, JR., W. F., BONILLA-RODRIGUEZ, G. O. Crystallization, preliminary X-ray diffraction analysis and Patterson search of oxyhaemoglobin I from the wolf (Chrysocyon brachiurus) Acta Cryst. D55, p. 1618-9, 1999. [ Links ]
SMARRA, A. L. S., DE AZEVEDO, JR., W. F., FADEL, V., DELATORRE, P., DELLAMANO, M., COLOMBO, M. F., BONILLA-RODRIGUEZ, G. O. Purification, crystallization, and preliminary X-ray analysis of hemoglobin-I from the armored catfish Liposarcus anisitsi. Acta Cryst. D56, p. 495-7, 2000. [ Links ]

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