Flow injection spectrophotometric determination of adrenaline using a solid-phase reactor containing triiodide ions immobilized in an anion-exchange resin
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Abstract
A flow injection spectrophotometric procedure with on-line solid-phase reactor containing
ion triiodide immobilized in an anion-exchange resin is proposed for the determination of adrenaline
(epinephrine) in pharmaceutical products. Adrenaline is oxidized by triiodide ion immobilized in an
anionic-exchange resin yielding adrenochrome which is transported by the carrier solution and detected
at a wavelength of 488 nm. Adrenaline was determined in three pharmaceutical products in the 6.4 x 10 -
6 to 3.0 x 10 -4 mol L -1 concentration range with a detection limit of 4.8 x 10 -7 mol L -1 . The recovery of this
analyte in three samples ranged from 96.0 to 105 %. The analytical frequency was 80 determinations per
hour and the RSDs were less than 1 % for adrenaline concentrations of 6.4 x 10 -5 and 2.0 x 10 -4 mol L -1
(n=10). A paired t-test showed that all results obtained for adrenaline in commercial formulations using
the proposed flow injection procedure and a spectrophotometric batch procedure agree at the 95%
confidence level.
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