Keywords
reflectance spectroscopy
hydrochlorothiazide
spot test
p-dimethylaminocinnamaldehyde
How to Cite
Abstract
This paper describes a method for quantitative spot test analysis of hydrochlorothiazide usingdiffuse reflectance spectroscopy. The reflectance measurements were performed analyzing the colored
compound (l = 585 nm) produced from the reaction between hydrochlorothiazide and p-
dimethylaminocinnamaldehyde (PDAC) in acid medium. This reaction occurred on filter paper after heating
to 80oC for 8 minutes. Factorial designs allowed varying multiple reaction factors simultaneously in order
to obtain the best reaction conditions. These factors included heating temperature, heating time, acid
volume and PDAC volume. The linearity was studied in the range of 3.36x10 -2 to 1.01x10 -1 mol L -1 with a
correlation coefficient of 0.998. The limit of detection was estimated to be 1.32x10 –2 mol L -1 . Commercial
samples were analyzed using the proposed method and the results were favorably compared with those of
the United States Pharmacopeia method, showing that quantitative spot test analysis by diffuse reflectance
could be successfully used to determine hydrochlorothiazide in medicines.
References
L. S. Goodman, A. Gilman, In The Pharmacological Basis
of Therapeutics, McGraw-Hill, New York, 10th ed., 1986,
chap. 29.
J. Ouyang, W. R. G. Baeyens, J. Delanghe, G. Van Der
Weken, W. Van Daele, D. De Keukeleire, A. M. García
Campaña, Anal. Chim. Acta 386(3) (1999) 257.
N. Erk, J. Pharm. Biomed. Anal. 24(4) (2001) 603.
O. Atay, U. Tamer, D. Arikan, Anal. Lett. 34(7) (2001) 1153.
N. Erk, F. Onur, Anal. Lett. 29(11) (1996) 1963.
I. E. Panderi, Pharm. Biomed. Anal. 21(1) (1999) 257.
I. E. Panderi, M. Parissi-Poulou, J. Pharm. Biomed. Anal.
(1) (1999) 1017.
M. C. F. Ferraro, P. M. Castellano, T. S. Kaufman, J.
Pharm. Biomed. Anal. 30(4) (2002) 1121.
N. Erk, Pharm. Biomed. Anal. 27(6) (2002) 901.
I. Albero, V. Ródenas, S. García, C. Sánchez-Pedreño,
J. Pharm. Biomed. Anal. 29(1-2) (2002) 299.
J. Joseph-Charles, S. Brault, C. Boyer, M. H. Langlois,
L. Cabrero, J. P. Dubost, Anal. Lett. 36(11) (2003) 2485.
T. Urbányi, A. O’Connell, Anal. Chem. 44(3) (1972) 565.
C. S. P. Sastry, T. N. V. Prasad, B. S. Sastry, E. V. Rao,
Analyst 113(1) (1988) 255.
J. Ouyang, W. R. J. Baeyens, J. Delanghe, G. Van Der
Weken, A. C. Calokerinos, Talanta 46(1) (1998) 961.
D. Kealey, Talanta 19(12) (1972) 1563.
R. Narayanaswamy, Analyst 118(4) (1993) 317.
S. G. Dmitrienko, O. A. Sviridova, L. N. Pyatkova, V. A.
Zhukova, Y. A. Zololov, Anal. Chim. Acta 405(1) (2000) 231.
F. A. A. Matias, M. M. D. C. Vila, M. Tubino, Sensors
and Actuators B 88(1) (2003) 60.
M. Tubino, A. V. Rossi, M. E. A. Magalhães, Anal. Lett.
(2) (1997) 271.
A. Ghauch, J. Rima, A. Charef, J. Suptil, C. Fachinger. M.
Martin-Bouyer, Talanta 48(1) (1999) 385.
Z. A. El Sherif, M. I. Walash, M. F. El-Tarras, A. O.
Osman, Anal. Lett. 30(10) (1997) 1881.
I. Hussain, U. Shaukat, J. Chem. Soc. Pakistan 24(4)
(2002) 282.
N. H. Zawilla, M. A. A. Mohammad, N. M., El Kousy, S. M.
El-Moghazy Aly, J. Pharm. Biomed. Anal. 27(1) (2002) 243.
A. Saeed, S. Haque, S. Z. Qureshi, Talanta 40(12) (1993)
B. A. Moussa, J. Pharm. Biomed. Anal. 23(6) (2000)
R. Agarwal, Am. J. Physiol.-Renal 283(2) (2002) F236.
M. Laudszun, K. Kovar, Pharm. Acta Helv. 66(9-10)
(1991) 268.
C. W. Nagel, Y. Glories, Am. J. Enol. Viticult. 42(4)
(1991) 364.
A. Ghauch, C. Turnar, C. Fachinger, J. Rima, A. Charef,
J. Suptil, M. Martin-Bouyer, Chemosphere 40(1) (2000) 1327.
J. C. Miller, J. N. Miller, Statistics for Analytical Chemis-
try, Ellis Horwood, Chichester, 2nd ed., 1992, chap. 5.
The United States Pharmacopeia, United States
Pharmacopeial Convention, Inc., Rockville, MD, 23rd ed.,
, 497.
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