Determination of hydrochlorothiazide in pharmaceutical formulations by diffuse reflectance spectroscopy
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Abstract
diffuse reflectance spectroscopy. The reflectance measurements were performed analyzing the colored
compound (l = 585 nm) produced from the reaction between hydrochlorothiazide and p-
dimethylaminocinnamaldehyde (PDAC) in acid medium. This reaction occurred on filter paper after heating
to 80oC for 8 minutes. Factorial designs allowed varying multiple reaction factors simultaneously in order
to obtain the best reaction conditions. These factors included heating temperature, heating time, acid
volume and PDAC volume. The linearity was studied in the range of 3.36x10 -2 to 1.01x10 -1 mol L -1 with a
correlation coefficient of 0.998. The limit of detection was estimated to be 1.32x10 –2 mol L -1 . Commercial
samples were analyzed using the proposed method and the results were favorably compared with those of
the United States Pharmacopeia method, showing that quantitative spot test analysis by diffuse reflectance
could be successfully used to determine hydrochlorothiazide in medicines.
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