Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

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Published: Mar 2, 2009
DOI: https://doi.org/10.26850/1678-4618eqj.v34.1.2009.p19-24
Keywords:
high performance liquid chromatography, maceration, microwave, ultrasonic, Zingiberaceae

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Cristiane Pimentel Victório
Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, Brasil.
https://orcid.org/0000-0002-2815-3725
Celso Luiz Salgueiro Lage
Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, Brasil.
https://orcid.org/0000-0002-3284-9469
Ricardo Machado Kuster
Universidade Federal do Rio de Janeiro, Laboratório de Fitoquímica, Rio de Janeiro, Brasil.
https://orcid.org/0000-0002-8961-5348

Abstract

The current study aims to verify the best method for a rapid and efficient extraction of flavonoids from Alpinia zerumbet. Dried leaves were extracted using distillated water and ethanol 70% by extraction methods of shaking maceration, ultrasonic, microwave and stirring. By the application of TLC and reversedphase HPLC techniques the rutin and kaempferol-3-O-glucuronide were detected. Ethanol 70% was more efficient for flavonoids extraction than water. No significant yielding variation was verified for ultrasonic, microwave and stirring methods using ethanol 70% (11 to 14%). The relative concentration of rutin and kaempferol-3-O-glucuronide, respectively, was higher by ultrasonic (1.5 and 5.62 mg g-1 dried leaves, respectively) and by microwave (1.0 and 6.64 mg g-1 dried leaves) methods using ethanol. Rapid and simplified extraction proceeding optimize phytochemical work and acquisition of secondary metabolites.

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How to Cite
Victório, C. P., Lage, C. L. S., & Kuster, R. M. (2009). Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents. Eclética Química, 34(1), 19–24. https://doi.org/10.26850/1678-4618eqj.v34.1.2009.p19-24
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Vol. 34 No. 1 (2009): Eclética Química
Section
Original articles
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