Spectrophotometric determination of nevirapine using tetrathiocyanatocobalt(II) ion as a reagent

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Thekke V. Sreevidya
Badiadka Narayana

Abstract

A simple and rapid spectrophotometric method for the determination of nevirapine is described. The method is based on the reaction of nevirapine with tetrathiocyanatocobalt(II) ion in buffer of pH 4 to form the corresponding complex. Beer’s law is obeyed in the range of 0.2 – 2.0 μg mL-1 for nevirapine. The optical parameters such as molar absorptivity, Sandell’s sensitivity, detection limit and quantitation limit were found to be 1.16× 104 Lmol-1cm-1, 2.09 X 10-3 μg cm-2, 0.073 μg mL-1 and 0.222 μg mL-1 respectively. The optimum reaction conditions and other analytical parameters were evaluated. The statistical evaluation of the method was examined by determining intra-day and inter-day precision. The proposed method has been successfully applied for the determination of nevirapine in pharmaceutical formulations.

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How to Cite
Sreevidya, T. V., & Narayana, B. (2010). Spectrophotometric determination of nevirapine using tetrathiocyanatocobalt(II) ion as a reagent. Eclética Química, 35(3), 93–102. https://doi.org/10.26850/1678-4618eqj.v35.3.2010.p93-102
Section
Original articles

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