Amperometric biosensor for ascorbic acid
Main Article Content
Abstract
extracted from the mesocarp of cucumber (Cucumis sativus) by using 0.05 mol L -1 phosphate buffer, pH
5.8 containing 0.5 mol L -1 NaCl. After the dialysis versus phosphate buffer
0.05 mol L -1 pH 5.8, the enzyme was immobilized onto nylon net through glutaraldehyde covalent
bond. The membrane was coupled to an O 2 electrode and the yielding reaction monitored by oxygen
depletion at -600 mV using flow injection analysis optimized to 0.1 mol L -1 phosphate buffer pH 5.8, as the
carrier solution and flow-rate of 0.5 mL min -1 . The ascorbic acid calibration curve was linear from 1.2x10 -4 to
1.0x10 -3 mol L -1 . The evaluation of biosensor lifetime leads to 500 injections. Commercial pharmaceutical
samples were analyzed with the proposed method and the results were compared with those obtained by
high-performance liquid chromatography (HPLC).
Metrics
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
The corresponding author transfers the copyright of the submitted manuscript and all its versions to Eclet. Quim., after having the consent of all authors, which ceases if the manuscript is rejected or withdrawn during the review process.
When a published manuscript in EQJ is also published in other journal, it will be immediately withdrawn from EQ and the authors informed of the Editor decision.
Self-archive to institutional, thematic repositories or personal webpage is permitted just after publication. The articles published by Eclet. Quim. are licensed under the Creative Commons Attribution 4.0 International License.
References
B. A. Fox, A. G. Cameron, Food Science, Nutrition and
Healths, 5th Edition, Edward Arnold, London, 1989, p. 261.
M. J. Villanueva, M. D. Tenorio, M. Sagardoy, A.
Redondo, M. D. Saco, Food Chem. 91(2005)609.
A.Hodgkinson, Oxalic acid in biological and medicine. Aca-
demic Press, London, 1977.
E. J. Oliveira, D. G. Watson, J. Chromatogr. A 764(2001)3.
H. J. Iwase, J. Chromatogr. A 881(2000)327.
A. Rizzolo, A. Brambilla, S. Valsecchi, P. Eccher-Zerbini,
Food Chem. 77(2002)257.
M. Rodríguez-Comsaña, M. S. García-Falcón, J. Siman-
Gándara, Food Chem. 79(2002)141.
W. M. Zeng, F. Martinuzzi, A. MacGregor, J. Pharm.
Biomed. Anal. 36(2005)1107.
L. Fernandez, H. Carrero, Electrochim. Acta
(2005)1233.
J. M. Zen, D. M. Tsai, A. S. Kumar, Electroanal.
(2003)1171.
J. Kulys, E. J. D’Costa, Anal. Chim. Acta 243(1991)173.
J. Kulys, A. Drungiliene, Electroanal. 3(1991)209.
M. Mascini, M. Iannello, G. Palleschi, Anal. Chim. Acta
(1983)135.
L. Macholán, B. Chmlíková, Anal. Chim. Acta 185(1986)187.
S. Uchiyama, Y. Umetsu, Anal. Chim. Acta 255(1991)53.
K. Matsumoto, K. Yamada, Y. Osajima, Anal. Chem.
(1981)1974.
E. Akyilmaz, E. Dinçkaya, Talanta 50 (1999) 87.
O. Fatibello-Filho, I.C. Vieira, J. Braz. Chem. Soc.
(4)(2000)412.
A. Manzoli, I. N. Tomita, F. L. Fertonani, G. Oliveira-
Neto, M. Mascini, H. Yamanaka, Anal. Lett. 37(2004)1823.
M. K. Sezgintürk, E. Dinçkaya, Bios. & Bioelect. 19
(2004) 835.
K. Rekha, M. D. Gouda, M. S. Thakur, N. G. Karanth,
Bios. & Bioelect. 15 (2000) 499.
R. F. Itzhaki, D. M. Gill, Anal. Biochem. 9(1964)401.
H. U. Bergmeyer, M. GrabL, H.E. Walter, Biochemical
reagents for general use. In: H. U. Bergmeyer (Ed.), Meth.
Enz. Anal. Weinheim: VCH Publishers, Inc., 1988, v. II, p. 157.
H. U. Bergmeyer (Ed.), Meth. Enz. Anal. Weinheim:
VCH Publishers, Inc., 1988, v. VI, p. 668.
I. A. Nicolson, R. Macrae, Analyst 109(1984)267.
H. M. A. B. Cardelo, M. A. C. Moraes, L. Cardelo, Alim.
Nutr. 5(1993/94)65.