Sensitive spectrophotometric determination of lamotrigine in bulk drug and pharmaceutical formulations using bromocresol green
Main Article Content
Abstract
Two new, simple, rapid and reproducible spectrophotometric methods have been developed for the determination of lamotrigine (LMT) both in pure form and in its tablets. The first method (method A) is based on the formation of a colored ion-pair complex (1:1 drug/dye) of LMT with bromocresol green (BCG) at pH 5.02±0.01 and extraction of the complex into dichloromethane followed by the measurement of the yellow ion-pair complex at 410 nm. In the second (method B), the drug-dye ion-pair complex was dissolved in ethanolic potassium hydroxide and the resulting base form of the dye was measured at 620 nm. Beer’s law was obeyed in the concentration range of 1.5-15 μg mL-1 and 0.5-5.0 μg mL-1 for method A and method B, respectively, and the = corresponding molar absorptivity values are 1.6932 x 104 and 3.748 x 104 L mol-1cm-1. The Sandell sensitivity values are 0.0151 and 0.0068 μg cm-2 for method A and method B, respectively. The stoichiometry of the ion-pair complex formed between the dug and dye (1:1) was determined by Job’s continuous variations method and the stability constant of the complex was also calculated. The proposed methods were applied successfully for the determination of drug in commercial tablets.
Metrics
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
The corresponding author transfers the copyright of the submitted manuscript and all its versions to Eclet. Quim., after having the consent of all authors, which ceases if the manuscript is rejected or withdrawn during the review process.
When a published manuscript in EQJ is also published in other journal, it will be immediately withdrawn from EQ and the authors informed of the Editor decision.
Self-archive to institutional, thematic repositories or personal webpage is permitted just after publication. The articles published by Eclet. Quim. are licensed under the Creative Commons Attribution 4.0 International License.
References
A.G. Gilman, J.G. Hardman, L.E. Limbird, “Goodman and Gilman’s the Pharmacological Basis of Therapeutics.” 10th ed. McGraw Hill, New York, U.S.A. (2001) pp. 539.
C. Sean, Sweetman, Martindale: The Complete Drug Reference 34th ed. Pharmaceutical Press, London. (2005) pp. 363.
M. Cociglio, R. Alric, O. Bouvier, J. Chromatogr. Biomed. Appl. 110: 1-2, J.Chromatogr. 572 (1991) 269.
G.L. Lensmeyer, B.E. Gidal, D.A. Wiebe, Ther. Drug Monit. 19 (1997) 292.
P. Angelis-Stoforidis, D.J. Morgan, T J. O’Brien, F.J.E. Vajda, J. Chromatogr. B, Biomed. Appl. 727 (1999) 113.
D. Croci, A. Salmaggi, U.de Grazia, G. Bernardi, Ther. Drug Monit. 23 (2001) 665.
M.M. Castel-Branco, A.M. Almeida, A.C. Falcao, T.A. Macedo, M.M. Caramona F.G.Lopez, J. Chromatogr. B, Biomed. Appl. 755 (2001) 119.
C.L. Cheng, C.H. Chouand, O.Y.P. Hu,. J. Chromatogr. B, Anal. Technol. Biomed. Life Sci. 817 (2005) 199.
K.M. Matar, P.J. Nicholls, S.A. Bawazir, M.I. Al-Hassan, A. Tekle, J. Pharm. Biomed. Anal. 17 (1998) 525.
D. Londero, P. Lo Greco, J. Chromatogr. B. Biomed. Sci. Appl. 691 (1997) 1 ISSN 1387-2273 139.
K.M. Patil, S.L. Bodhankar, J. Chromatogr. B, 823 (2005) 152.
J. Hallbach, H. Vogel, W.G. Guder, Eur. J. Clin. Chem. Clin. Biochem. 35 (1997) 755.
E. Dreassi, G.P. Corbini, M. Corti, Ulivelli, R. Rocchi,. J. AOAC. Intl. 79: 6, ISSN 1060-3271 (1996) 1277.
N.F. Youssef, E.A. Taha,..Chem. Pharm. Bull. 55 (2007) 4 541.
G-S. Elizabeth, S. Giannoutsos, D.R. Lower, M.A. Virji, M.D. Krasowski, J. Chromatogr. Sci. 45 (2007) 9 616.
Z.K. Shihabi, K.S. Oles, J. Chromatogr. B, Biomed Appl. 683 (1996) 119.
R. Theurillat, M. Kuhn, W. Thormann, J. Chromatogr. A. 979 (2002) 353.
R.A. Biddlecombe, K.L. Dean, C.D. Smith, S.C. Jeal,. J. Pharm. Biomed. Anal. 8 (1990) 691.
J.M. Sailstad. J.W.A. Findlay, Ther. Drug. Monit., 13 (1991) 433.
R.S. Talekar, A.S.Dhake, D.B. Sonaje, V.K. Mourya, Ind. J. Pharm. Sci. 62 (2000) 51.
D-R. Olga, M.E Calvo, M. Acros-Martinez, J.. Sensors. 8 (2008) 4201; DOI:10.3390/s8074201
N. Rahman, S.N. Hejaz-Azmi, J. Pharm. Biomed. Anal. 24 (2000) 33.
N. Silva, E.E.S. Schapoval, J. Pharm. Biomed. Anal. 29 (2002) 749.
T. Perez-Ruiz, C. Martinez-Lozano, V. Tomás, A. Sanz, E. Sauquillo, J. Pharm. Biomed. Anal. 26 (2001) 609.
K. Basavaiah, V.S. Charan, IL Farmaco. 57 (2002) 9. [26] H. Maghsoudi, A. Fawzi, J. Pharm. Sci. 67 (1978) 32.
H. Farsam, H.H. Yahya-saeb, A. Fawzy, Int. J. Pharm. 7 (1981) 343.
A.S. Douglas, M.W. Donald. “Principels of Instrumental Analysis,” Holt, Rinhart. and Winston, New York, 1971.pp. 104.
N. Erk. Anal. Lett. 36 (2003) 1183.
H. Zavis, D. Ludvik, K. Milan, S. Ladislaw, V. Frantisck, Handbook of Organic Reagents in Inorganic Analysis. Translated by Stanislav, K, Dr. Chalmers (The Series and Translation Editor: University of Aberdem, Ellis Horwood Limited, Chichester, A Division of John Wiley & Sons IC, New York, London, Sydney, Toronto.1976. pp.364.
International Conference on Hormonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2(R 1),Complementary Guideline on Methodology dated 06 November 1996, incorporated in November 2005, London.
J. Inczedy, T. Lengyel, A.M. Ure,. IUPAC Compendium of Analytical Nomenclature : 1998. Definitive Rules, Blackwell Science Inc., Boston.