Separation of lactic acid and by-products obtained by catalytic conversion of glycerol using high-performance liquid chromatography

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Published: Apr 6, 2022
DOI: https://doi.org/10.26850/1678-4618eqj.v47.2.2022.p74-81

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Andreza de Faria Alves Cruz
Federal University of Rio de Janeiro, Chemical and Biochemical Process Engineering Program/School of Chemistry, Rio de Janeiro, Brazil.
https://orcid.org/0000-0003-1321-3024
Gabriella Pinho Dias
Federal University of Rio de Janeiro, Chemical and Biochemical Process Engineering Program/School of Chemistry, Rio de Janeiro, Brazil.
https://orcid.org/0000-0002-7170-6743
Flávia de Rezende Bittencourt
Federal University of Rio de Janeiro, Chemical and Biochemical Process Engineering Program/School of Chemistry, Rio de Janeiro, Brazil.
https://orcid.org/0000-0001-5096-7111
Donato Alexandre Gomes Aranda
Federal University of Rio de Janeiro, Chemical and Biochemical Process Engineering Program/School of Chemistry, Rio de Janeiro, Brazil.
https://orcid.org/0000-0002-5736-4118

Abstract

Lactic acid is an attractive raw material in synthesizing many products. A new method for quantifying glycerol, lactic acid, and the by-products (pyruvaldehyde) obtained in this reaction was developed using high-performance liquid chromatography (HPLC) with a refractive index detector (HPLC-RI) in a column (300 × 7.7 mm, 8 µm) using H2SO4 0.001 M + 10% ACN (organic modifier) as mobile phase (0.6 mL min–1). This method indicated outstanding linearity for glycerol and lactic acid concentration from 0.6 to 6.6 g L–1 (coefficient of determination (R²) = 0.9912 and 0.9961, respectively) and accuracy between 98.33 and 100.00%. From this, it was possible to conclude that the method is applicable and concise for separating the primordial products in this reaction.

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How to Cite
Cruz, A. de F. A., Dias, G. P., Bittencourt, F. de R., & Aranda, D. A. G. (2022). Separation of lactic acid and by-products obtained by catalytic conversion of glycerol using high-performance liquid chromatography. Eclética Química, 47(2), 74–81. https://doi.org/10.26850/1678-4618eqj.v47.2.2022.p74-81
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Vol. 47 No. 2 (2022): Eclética Química Journal
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Original articles
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