Larvicidal activity, molluscicide and toxicity of the essential oil of Citrus limon peels against, respectively, Aedes aegypti, Biomphalaria glabrata and Artemia salina

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Published: Oct 1, 2019
DOI: https://doi.org/10.26850/1678-4618eqj.v44.4.2019.p85-95

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Paulo Roberto Barros Gomes
Instituto Federal do Pará- Campus Abaetetuba
http://orcid.org/0000-0002-4221-6577
Marlucy Bezerra Oliveira
UNIVERSIDADE FEDERAL DO MARANHÃO
Dionney Andrade de Sousa
http://orcid.org/0000-0003-4788-036X
Jeremias Caetano da Silva
Romer Pessôa Fernandes
Hilton Costa Louzeiro
Universidade Federal do Maranhão
http://orcid.org/0000-0001-7479-5879
Rayone Wesley Santos de Oliveira
Universidade Federal do Maranhão
http://orcid.org/0000-0001-6430-2225
Maria do Livramento de Paula
Universidade Federal do Maranhão
http://orcid.org/0000-0002-9552-3693
Victor Elias Mouchrek Filho
Universidade Federal do Maranhão
http://orcid.org/0000-0003-2855-7292
Maria Alves Fontenele
Universidade Federal do Maranhão- Imperatriz
http://orcid.org/0000-0003-0780-7563

Abstract

In this present work, we tested the larvicidal activity, molluscicide and toxicity of the oil extracted from Citrus limon peels, respectively against third stage larvae of Aedes aegypti, snail Biomphalaria glabrata, and Artemia salina. For this, we extract the essential oil by hydrodistillation. Then, we identified and quantified the components by gas chromatography coupled to mass spectrometry (GC-MS). We tested the larvicidal and molluscicidal activity, respectively, using the method adopted by the Brazilian Ministry of Health and the World Health Organization. We calculated the lethal concentration (LC50) from the Probit method for the three biological activities with 95 %. The results of the chromatographic analysis showed that the oil has 58.81% of Limonene (major constituent) and 0.11% α-Mulene (minority component). The essential oil presented lethal concentration (LC50) for larvicidal activity, molluscicide and toxicity, respectively at 15.48, 13.05 and 743.35 mg∙L-1. Therefore, the essential oil is active against larvae of A. aegypti and snail B. glabrata and non-toxic against larvae of A. salina.

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Barros Gomes, P. R., Oliveira, M. B., de Sousa, D. A., da Silva, J. C., Fernandes, R. P., Louzeiro, H. C., de Oliveira, R. W. S., de Paula, M. do L., Mouchrek Filho, V. E., & Fontenele, M. A. (2019). Larvicidal activity, molluscicide and toxicity of the essential oil of Citrus limon peels against, respectively, Aedes aegypti, Biomphalaria glabrata and Artemia salina. Eclética Química, 44(4), 85–95. https://doi.org/10.26850/1678-4618eqj.v44.4.2019.p85-95
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Vol. 44 No. 4 (2019): Eclética Química Journal
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Original articles

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