How to Cite
Abstract
Empregou-se nesse trabalho alguns vegetais como fonte de peroxidase. Após a determinação de proteína total, atividade e atividade específica, selecionou-se o extrato bruto da abobrinha (Cucurbita pepo) como fonte dessa enzima para ser empregado em um sistema de análise por injeção em fluxo com detecção espectrofotométrica para a determinação de diversos compostos fenólicos (e.g. fenol, catecol, 2,4-diclorofenol, 4-cloro-3-metilfenol, 4-acetamidofenol, 4-clorofenol, 2,4,6-triclorofenol, o-cresol, m-cresol, p-cresol e hidroquinona). Após a otimização desse sistema em fluxo, o mesmo foi empregado na determinação de compostos fenólicos em águas residuárias de indústrias da região de São Carlos-SP, no intervalo de concentração de 2,0x10-4 a 4,0x10-3 mol L-1, com LD de 8,0x10-5 mol L-1 e freqüência analítica de 58 h-1. A recuperação de fenol em 3 amostras variou de 98,3 a 106, 2% e o RSD foi menor que 1,2% para soluções de fenol nas concentrações de 6,0x10-4 e 8,0x10-4 mol l-1 (n=10).
References
CARUSO, C. S.; VIEIRA, I. C.; FATIBELLO-FILHO, O. Determination of epinephrine and dopamine in pharmaceutical formulations using a biosensor based on carbon paste modified with crude extract of cara root (Dioscorea bulbifera). Anal. Lett., v.32, p.39-50, 1999. [ Links ]
Companhia Estadual de Technologia e Saneamento Básico (CETESB). Determinação de fenóis em águas – método colorimétrico da 4-aminoantipirina. São Paulo: 1990. 125p. [ Links ]
DIXON, M.; WEBB, E.C. Enzymes. New York: Academic Press. p. 3-44, 1979. [ Links ]
EATON, A. D.; CLESCERI, L.S.; GREENBERG, A. E. Standard methods for the examination of water and wastewater. 19.ed. Washington: American Public Health Association, American Water Works Association and Water Environment Federation. p. 5-36, 1995. [ Links ]
FATIBELLO-FILHO, O.; VIEIRA, I. C. Construction and analytical aplication of a biosensor based os stearic acid-graphite powder modified with sweet potato tissue in organic solvents. Fres. J. Anal. Chem., v.368, p.338-342, 2000. [ Links ]
FATIBELLO-FILHO, O.; VIEIRA, I. C. Flow injection spectrophotometric determination of sulfite using a crude extract of sweet potato root (Ipomoea batatas (L.) Lam.) as a source of polyphenol oxidase. Anal. Chim. Acta, v.354, p.51-55, 1997. [ Links ]
FATIBELLO-FILHO, O.; VIEIRA, I. C. L-Ascorbic acid determination in pharmaceutical formulations using a biosensor based on carbon paste modified with crude extract of zucchini (Cucurbita pepo). J. Braz. Chem. Soc., v.11, p.412-418, 2000. [ Links ]
KHALAF, K.D.; HASAN, B.A.; MORALES-RUBIO, A.; LA-GUARDIA, M. Spectrophotometric determination of phenols with sodium metaperiodate and aminophenols, Talanta, v.29, p.917-920, 1982. [ Links ]
MANAHAN, S.E. Fundamentals of enviromental chemistry. Tokyo: Lewis Publishers. 1993. 756p. [ Links ]
MOHLER, E. F.; JACOB, L.N. Determination of phenolic-type compounds in water and industrial wastewater: comparison of analytical methods. Anal Chem, v.29, p.1369-1371, 1957. [ Links ]
MÖLLER, J.; MARTIN, M. Determination of phenol in water by flow injection analysis. Fres. Z. Anal. Chem., v.329, p.728-731, 1988. [ Links ]
VIEIRA, I. C.; FATIBELLO-FILHO, O. Amperometric biosensor for the determination of phenols using a crude extract of sweet potato (Ipomoea batatas (L.) Lam.). Anal. Lett., v.30, p.895-907, 1997. [ Links ]
VIEIRA, I. C.; FATIBELLO-FILHO, O. Biosensor based on paraffin/graphite modified with sweet potato for the determination of hydroquinone in cosmetic cream in organic phase. Talanta, v.52, p.681-689, 2000. [ Links ]
VIEIRA, I. C.; FATIBELLO-FILHO, O. Flow injection spectrophotometric determination of total phenols using a crude extract of sweet potato root (Ipomoea batatas (L.) Lam.) as enzymatic source. Anal. Chim. Acta, v.366, p.111-118, 1998. [ Links ]
VIEIRA, I. C.; FATIBELLO-FILHO, O. L-Cysteine determination using a polyphenol oxidase-based inhibition flow injection procedure. Anal. Chim. Acta, v.366, p.287-293, 1998. [ Links ]
VIEIRA, I. C.; FATIBELLO-FILHO, O. Spectrophotometric determination of methyldopa and dopamine in pharmaceutical formulations using a crude extract of sweet potato root (Ipomoea batatas (L.) Lam.) as enzymatic source. Talanta, v.46, p.559-564, 1998. [ Links ]
VIEIRA, I. C.; FATIBELLO-FILHO, O.; ANGNES, L. Zucchini crude extract-palladium-modified carbon paste electrode for the determination of hydroquinone in photographic developers. Anal. Chim. Acta, v.398, p.145-151, 1999. [ Links ]
VIEIRA, I. C.; FATIBELLO-FILHO, O. Flow injection spectrophotometric determination of hydrogen peroxidase using a crude extract of zucchini (Cucurbita pepo) as a source of peroxidase. Analyst, v.123, p.1809-1812, 1998. [ Links ]
WHITAKER, J.R. Principles of enzymology for the food science. New York: Marcel Dekker. p.45-59, 1972. [ Links ]
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2018 Eclética Química Journal